• 500 MHz NMR metabolomics

    quantitative

Listing Row

Monday, June 8, 2015
Monday, June 8, 2015
  • Sample Submission

    The NMR Core routinely runs quantitative untargeted metabolomics on whole blood, serum, plasma, urine, cell media. Please feel free to contact Larisa Yeomans yeomans@umich.edu or Kathleen Stringer stringek@med.umich.edu with questions about your samples and study planning, and when possible, prior to your sample collection.  NMR metabolomics is a powerful platform but not very sensitive, hence ideally we need a minimum volume of 600uL of biofluid and when possible one or two technical replicates.

  • Sample process

    After a conversation on the feasibility of running your samples by NMR, we will send you a quote. Upon your acceptance and provision of a short code, you may submi your samples your samples (please bring them in dry ice), and update their information in our freezers using the university freezer inventory 'Freezerworks'. We will process your samples according to the standard operating procedure (SOP) used for your biofluid (precipitation/extraction/lyophilization/filtration) and an internal standard or a known concentration (DSS-d6 or Formate) will be added.  Metabolomics samples are run on the 500MHz NMR instrument in our Core.

  • Results

    After spectral acquisition, the NMR spectra is processed and profiled using commercially available Chenomx software which contains a library of hundreds of metabolites and each detectable metabolite is profiled to yield a metabolite concentration.  At the end you will have a list of metabolites (30-50 metabolites depending on the biofluid) and their concentration in uM units.

  • Fees

    University of Michigan investigators:  $163 per sample

    External investigators: $210 per sample plus the % IDC